Introduction: Whole Body Vibration (WBV) as a new method and supplement for neuromuscular training, cause mechanical stimulation in this system. Propose: The aim of present study was to determine the effect of Whole Body Vibration training (WBVT) on some factors of the physical fitness, growth hormone and IGF-1 concentration in trained girl students. Materials and methods: twenty female of physical education students with a mean and standard deviation of age (21.75 ± 2.2 Yr.), weight (54.25 ± 7.28 kg) and BMI (20.1± 2.44 kg/m2) were selected and randomly divided into two groups: experimental (n=10) and control (n=10). WBVT for 10 days with frequencies 30 Hz and amplitude of 10 mm in six different body positions were performed. Blood samples were collected 24 hours before and after WBVT. Data were analyzed by dependent t test and paired t test. Results: The statistical analysis showed, WBVT lead to significant increase of strength, speed, power, growth hormone and IGF-1 concentrations in experimental group (P<0.05), while agility in this group was not significantly changed (P>0.05). Conclusion: WBV training program can be used as modern training methods and supplements along with other training methods to improve the levels of anabolic hormones and physical fitness factors.