پژوهش در طب ورزشی و فناوری

تأثیر گرم‌کردن با دو شدت متفاوت بر میزان پلاسمایی آنزیم‌های ضد اکسایشی و شاخص‌های تخریب لیپیدی، پروتئینی و دی.ان.ای بعد از فعالیت شدید در دانشجویان غیرورزشکار

نویسندگان

محمدرضا دهخدا
کاظم خدایی
سهراب ملک زاده
چکیده
هدف این پژوهش بررسی تأثیر گرم­کردن با دو شدت متفاوت بر میزان پلاسمایی آنزیم­های ضد اکسایشی و شاخص­های تخریب لیپیدی، پروتئینی و دی.ان.ای بعد از فعالیت شدید در دانشجویان غیرورزشکار بود. 12 نفر از دانشجویان غیرورزشکار دانشگاه خوارزمی برای پژوهش انتخاب شدند. ابتدا میزان VO2peakآزمودنی­ها با دستگاه گازآنالیزر روی چرخ کارسنج اندازه­گیری و میزان کار در شدت­های کم و زیاد گرم­کردن محاسبه شد. سپس آزمودنی­ها در سه جلسه با فاصله 48 ساعت به سه گروه گرم­کردن با شدت کم و گرم­کردن با شدت زیاد و بدون گرم کردن تقسیم شدند. در ابتدای هرجلسه خون­گیری استراحتی انجام شد. سپس، گروه اول گرم­کردن با شدت کم (%50-%45 VO2peak) را به­مدت شش­دقیقه انجام دادند سپس اجرای فعالیت شدید آغاز شد و گروه دوم گرم­کردن با شدت زیاد (%80-%75 VO2peak) و سپس فعالیت شدید را انجام دادند و گروه سوم مستقیم بدون گرم­کردن فعالیت شدید را انجام دادند. دو ساعت پس از فعالیت شدید خون­گیری صورت گرفت. در روزهای دیگر به­صورت متقاطع جای اعضای گروه­ها عوض ­شد. از تحلیل واریانس اندازه­گیری­های مکرر و آزمون تعقیبی LSDبرای تجزیه و تحلیل آماری استفاده شد. یافته­های پژوهش نشان داد که بین میانگین تغییرات MDA، کربونیل-پروتئین و 8-هیدروکسی 2-دزوکسی گوانوزین و همچنین بین میانگین تغییرات آنزیم­های ضد اکسایشی SOD، CAT و GPX نسبت به حالت استراحتی در سه حالت تفاوت معنی­داری وجود داشت. میانگین تغییرات MDA و SOD در حالت گرم­کردن با شدت کم کمتر از حالت­های دیگر بود. ولی این کاهش معنی­دار نبود. متغیرهای دیگر در حالت بدون گرم­کردن کمترین مقدار و در گرم­کردن شدید بیشترین مقدار را داشتند. نتایج پژوهش نشان داد که گرم­کردن با شدت کم می­تواند تخریب لیپیدی را کاهش دهد، ولی بر تخریب پروتئینی و DNA اثری ندارد. گرم­کردن با شدت زیاد نیز می­تواند باعث افزایش هر سه نوع تخریب شود. بنابراین در مردان غیرورزشکار گرم­کردن با شدت کم بهتر از شدت زیاد است.

کلیدواژه‌ها

گرم‌کردن
آنزیم‌های ضد اکسایشی
تخریب لیپیدی
تخریب پروتئینی
تخریب DNA
فعالیت شدید

عنوان مقاله English

Effect of different intensity warm up on plasma antioxidant enzymes and damage indices of lipid, protein and DNA after intensive activity in non-athlete males

نویسندگان English

MohammadReza Dehkhoda
Kazem Khodai
Sohrab Malekzadeh
چکیده English

The aim of this research was to study the effect of warm up with two different type intensity on plasma antioxidant enzymes and damage indices of lipid, protein and DNA after intensive activity in non-athlete males. 12 non athlete students of kharazmi University participated in this study. Firist, subjects VO2peak were measured by Gas analyzer (Meta Max 3B) on the ergometer cycle with incremental test. Work rate in the low and high intensities of warm up were calculated for each subjects on the VO2peak chart Then subjects in the three sessions with 48 hours interval divided to three groups of warm up with low and high intensity and non-warm. In each session resting blood sampling collected then first group warm up with low intensity (45-50% VO2peak) for six minutes and then perform intensive activity The Second group performed warm up with high intensity (75-80% VO2peak) and then intensive activity and third group directly without warm up performed intensive activity. Blood sampling collected after two hours of the intensive activity. In other sessions group members were changed by cross design method. Using analysis of variance with repeated measuring and the LSD test for statistic analyze. The results showed a significant differences in MDA, carbonyl-protein 8-hydroxy-2-deoxy Guanosine and also antioxidant enzymes SOD, CAT and GPX companing to resting state. Main difference of MDA and SOD was not signifacnatly lower in the low intensity warm up state rather than other state. In the other variables non warm up state have lower value and intensity warm up state have higher value. Result of the study indicate that low intensity warm up can decreased lipid damage but not effected on the protein and DNA damage. Also, High intensity warm up can case increases all three type of damage. Therefore low intensity warm up in non-athlete males is better than the high intensity warm up.

کلیدواژه‌ها English

Warm Up
antioxidant enzymes
Lipid Damage
Protein Damage
DNA Damage
Intensive Activity
1. Boveris, A., Chance, B. (1973). The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochemical Journal. 134: 707-16.
2. Cooper, C.E., Vollaard, N.B., Choueiri, T., Wilson, M.T. (2002). Exercise, free radicals and oxidative stress. Biochemical Society Transactions. 30(2): 280-4.
3. Jackson, M.J., Khassaf, M., Vasilaki, A., Mcardle, F. (2005).Vitamin E and the oxidative stress of exercise. Annals of the New York Academy of Sciences. 1031:158-65.
4. Dornelles, C., Oliveire, A. R (2004). Oxygen free radical exercise: mechanisms of synthesis and adaption training. Revista Brasileira de Medicina do Esporte. VOL. 10. NO4, PP: 308-13.
5. McCord, J.M (1985). Oxygen-derived free radicals in post ischemic tissue injury. The New England Journal of Medicine. 312:159-63.
6. Antony, M., James, T. (2010). A study on the effect of isobaric hypoxia on antioxidant enzyme activity and lipid peroxidation in rat brain. International Journal of Pharmaceutical Sciences and Research. 1(9): 67-75.
7. Burnley, M., Jones A.M., Carter, H., Doust, J.H. (2000).Effects of prior heavy exercise on phase II pulmonary oxygen uptake kinetics during heavy exercise. Journal of Applied Physiology. 89: 1387–96.
8. Burnley, M., Doust, J.H., Jones, A.M. (2006). Time required for the restoration of normal heavy exercise VO2 kinetics following prior heavy exercise. Journal of Applied Physiology. 101(5): 1320-7.
9. Gerbino, A., Ward, S.A., Whipp, B.J. (1996). Effects of prior exercise on pulmonary gas-exchange kinetics during high- intensity exercise in humans. Journal of Applied Physiology. 80: 99–107.
10. Burnley, M., Jones, A.M. (2007). Oxygen uptake kinetics as a determinant of sports performance. European Journal of Sport Science. 7(2): 63-79.
11. Nicole, D.P., Kowalchuk, J.M., Peterson, D.H. (2005).Effect of prior heavy- intensity exercise during single leg knee-extension on vo2 kinetics and limb blood flow. Journal of Applied Physiology. 99: 1462–70.
12. Nazarali, P., Rezayi, N. (2010). Effect of warm up intensity below the lactate threshold on vo2 slow component during submaximal exercise in elite futsall players. Middle-East Journal of Scientific Research. 5(4):252-5.
13. Mandengue, H.S., Miladi, I., Bishop, D., Temfemo, A., Cisse, F., Ahmaidi, S. (2009). Methodological approach for determining optimal active warm-up intensity: predictive equations. Science & Sports. 24: 9–14.
14. Robertson, A., Watt, J.M., Galloway, S.D.R. (2004).Intensity cycling exercise effects of leg massage on recovery from high. British Journal of Sports Medicine. 38:173-6.
15. Bailey, S.J., Vanhatalo, A., Wilkerson, D.P., DiMenna, F.J., Jones, A.M. (2009). Optimizing the “priming” effect: influence of prior exercise intensity and recovery duration on O2 uptake kinetics and severe-intensity exercise tolerance. Journal of Applied Physiology. 107(6): 1743-56.
16. Steven, H.Y., Joseph A.K., Sidney, M.H. (1978). Lipid peroxides in plasma as measured by liquid-chromatographic separation of malondialdehyde Thiobarbituric acid adduct. Clinical Chemistry. 32: 214-20.
17. Reznick, A.Z., Packer, L. (1994). Oxidative damage to proteins: spectrophotometeric method for carbonyl assay. In: Packer, L. (Ed), Methods in Enzymology. Academic press, San Diego CA. 233: 363-75.
18. Irie, M., Asami, S., Nagata, S., Ikeda, M., Miyata, M., Kasai, H. (2001). Psychosocial factors as a potential trigger of oxidative DNA damage in human leukocytes. Japanese Journal of Cancer Research. 92(3): 367-76.
19. Flohe, L., Otting, F. (1984).Superoxide dismutase assay. Methods in Enzymology. 105: 93-104.
20. Abei, H. (1984). Catalase in vitro. Methods in Enzymology. 105:121-6.
21. Paglia, D.E., Valentine, W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine. 70:158-65.
22. Altan, M., Atukeren, P., Mengi, M., Metin, M., Cakar, L., Gumustas, K. (2009). Influence of intermittent hypobaric exposure on SOD and TBARS levels in trained rats. Chinese journal of physiology. 52(2): 106-12.
23. Antony, M., James, T. (2010). A study on the effect of isobaric hypoxia on antioxidant enzyme activity and lipid peroxidation in rat brain. International Journal of Pharmaceutical Sciences and Research. 1(9): 67-75.
24. Clanton, T.L. (2007). Hypoxia-induced reactive oxygen species formation in skeletal muscle. Journal of Applied Physiology. 102: 2379–88.
25. Buchheit, M., Laursen, P.B., Ahmaidi, S. (2009). Effect of prior exercise on pulmonary O2 uptake and estimated muscle capillary blood flow kinetics during moderate-intensity field running in men. Journal of Applied Physiology. 107(2): 460-70.
26. Geor, R. J., McCutcheon, L.J., Hinchcliff, K.W. (2000). Effects of warm-up intensity on kinetics of oxygen consumption and carbon dioxide production during high-intensity exercise in horses. American Journal of Veterinary Research. 61(6):638-45.
27. Jones, A.M., DiMenna, F., Lothian, F., Taylor, E., Garland, S.W., Hayes, P.R., Thompson, K.G. (2008). ‘Priming’ exercise and O2 uptake kinetics during treadmill running. Respiratory Physiology & Neurobiology. 161: 182–8.
28. Sen, C., PacKer, L., Hanninen, O. (2000). Handbook of oxidant and antioxidants in exercise. Amesterdam: Elsevier: 89-114.
29. McCord, J.M. (1993). Free radical and oxidant balance. Clinical Biochemistry. 26 (5):351-7.
30. Beketic-Oreskovic, L., Ozretic, P., Rabbani, Z.N., Jackson, I.L., Sarcevic, B., Levanat, S., Maric, P., Babic, I.,Vujaskovic, Z. (2011). Prognostic significance of carbonic anhydrase IX (CA-IX), endoglin (CD105) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in breast cancer patients. Pathology & Oncology Research. 17(3): 593-603.
31. Pansarasa, F., Bertorelli, O., Valle, L., Della, .G (1997). Blood free radical oxidant enzymes and lipid peroxidant following long–distance and lactacidemic performance in highly trained aerobic and sprint athlete. Journal of Sports Medicine and Physical Fitness. 37:235-9.
32. Poulsen, H.E., Weimann, A., Loft, S. (1999). Methods to detect DNA damage by free radical: relation to exercise. The Proceedings of the Nutrition Society[Journal]. 58 (4):1007-14.
33. Ji, L.L., Gomez-Cabrera, M.C., Steinhafel, N., Vina, J. (2004). Acute exercise activates nuclear factor (NF)-κappaB signaling pathway in rat skeletal muscle. Federation of American Societies for Experimental Biology Journal. 18(13): 1499-506.
34. Ravi-Kiran, T., Subramanyam, M.V., Prathima, S., Asha, D.S. (2006). Blood lipid profile and myocardial superoxide dismutase in swim-trained young and middle-aged rats: comparison between left and right ventricular adaptations to oxidative stress. Journal of Comparative Physiology B. 176: 749-62.