Research in Sports Medicine and Technology

Effect of acute aerobic activity after a period of taking vitamin C supplement on Iron status and hematological indices of professional football players

Authors

Majid Mardaniyan Ghahfarrokhi
Abdolhamid Habibi
Hamed Rezaei Nasab

Shahid Chamran University, Iran

Abstract
Vitamin C consumption is important in Iron absorption. Therefore, the purpose of this study was to investigate the effect of acute aerobic activity after two weeks vitamin C supplementation on Iron status and hematological indices in club football players. In this quasi-experimental study, 40 club football players from Khuzestan province were randomly divided into two groups of vitamin C and placebo. Daily and for 14 days, subjects received 400 mg vitamin C in vitamin C group and 400mg dextrose in placebo group. 48 hours after the end of supplementation, a Bruce test was performed. To measure Iron, Ferritin, RBC and Hb levels, four blood samples were taken from the subjects, before and after the supplementation, immediately after and 72 hours after the Bruce test. Repeated measure ANOVA and Two way analysis of variance was used. 2 weeks of vitamin C supplementation showed a significant increase in RBC (p=0.026), Hb (p=0.041), serum Iron (p=0.008) and serum Ferritin (p=0.004) compared to placebo group. After aerobic exhaustive activity, RBC (p=0.034), serum Iron (p=0.023) and Ferritin (p=0.031) in vitamin C group significantly decreases compared to placebo group. After 72 hours of recovery, the vitamin‌ C group showed a significant increase in serum Iron (p=0.036) and Ferritin (p=0.045) compared to placebo group. It seems that two weeks of vitamin C supplementation can improve the serum Iron, Ferritin, RBC and Hb profile of professional footballers before aerobic exhaustive activity and in recovery.

Keywords

Physical Activity
Football
Anemia
ascorbic acid
1. Lashkari, F., Samavati Sharif, M.A., Ranjbar, K. (2016). The effect of two different modes of exercise swimming and vitamin C supplementation on anemia indices in male wistar rat. Journal of Knowledge & Health. 11(1):55-61.
2. Skarpańska-Stejnborn, A., Basta, P., Trzeciak, J., Szcześniak- Pilaczyńska, Ł. (2015). Effect of intense physical exercise on hepcidin levels and selected parameters of iron metabolism in rowing athletes. European Journal of Applied Physiology. 11(5):345-51.
3. Pouramir, M., Haghshenas, O., Sorkhi, H. (2015). Effects of gymnastic exercise on the body iron status and hematologic profile. Iranian Journal of Medical Sciences, 29(3):140-1. (Persian)
4. Moll, R., Davis, B. (2017). Iron, vitamin B12 and folate. Medicine. 45(4):198-203.
5. Moretti, D., Goede, J. S., Zeder, C., Jiskra, M., Chatzinakou, V., Tjalsma, H., Melse-Boonstra, A., Brittenham, G., Swinkels. D.W., Zimmermann, M.B. (2015). Oral iron supplements increase hepcidin and decrease iron absorption from daily or twice-daily doses in iron-depleted young women. Blood. 126(17):1981-9.
6. Anschuetz, S., Rodgers, C.D., Taylor, A.W. (2010). Meal composition and iron status of experienced male and female distance runners. Journal of Exercise Science & Fitness. 8(1):25-33.
7. Elgushy, E.E. (2016). Iron status for the Egyptian female players in running competition (short-middle-long distances)– comparative study. Ovidius University Annals, Series Physical Education and Sport / Science, Movment and Health, 16:449-53.
8. Sim, M., Dawson, B., Landers, G., Swinkels, D.W., Tjalsma, H., Trinder, D., P, Peeling. (2013). Effect of exercise modality and intensity on post-exercise interleukin-6 and hepcidin levels. International Journal of Sport Nutrition and Exercise Metabolism. 23(2):178-86.
9. Silva, A.S., Santhiago, V., Papoti, M., Gobatto, C.A. (2008). Hematological parameters and anaerobic threshold in Brazilian soccer players throughout a training program. International Journal of Hematology. 30(2):158-66.
10. Sporiš, G., Vlahović, T., Trajković, N., Milanović, Z., Madić, D. (2016). Hematological and iron status following a soccer match. Facta Universitatis. Series: Physical Education and Sport. 14(2):289-95.
11. Joksimović, A., Stanković, D., Ilić, D., Joksimović, I., Jerkan, M. (2009). Hematological profile of Serbian youth national soccer teams. Journal of Human Kinetics. 22:51-9.
12. Ostojic, S.M., Ahmetovic, Z. (2009). Indicators of iron status in elite soccer players during the sports season. International Journal of Laboratory Hematology. 31(4): 447-52.
13. Kılınç, F. (2010). Effect of vitamin C and E combination on hormonal, enzymatic and hematological values in blood of forced training basketball players. Biology of Sport. 27(1).
14. Lane, D.J., Richardson, D.R. (2014). The active role of vitamin C in mammalian iron metabolism: much more than just enhanced iron absorption. Free Radical Biology and Medicine. 75:69-83.
15. Bourque, S.P., Pate, R.R., Branch, J.D. (1997). Twelve weeks of endurance exercise training does not affect iron status measures in women. Journal of the American Dietetic Association. 97(10):1116-21
16. Dabbagh Nikookheslat, S. (2008). The effect of 12 weeks resistance training on resting levels and responses of hemoreological and coagulant variables to a session of resistance activity in men. PhD thesis. Faculty of Physical Education and Sport Sciences, University of Tehran.
17. Montagnana, M., Salvagno, G.L., Lippi, G. (2009). Circadian variation within hemostasis: an underrecognized link between biology and disease? Journal of Thrombosis and Hemostasis. 35(1):23-3.
18. Sari Sarraf, V., Asri Rezaei, S., Amirsasan, R., Zolfeghar-didani, H. (2013). The effects of vitamin C and E supplementation and anaerobic activity on oxidative indices in male teenager speed skaters. Olympic Quarterly. 63(3):7-18.
19. Willett, W. (2012). Nutritional Epidemiology: Oxford University Press.
20. Daneshvar, P., Hariri, M., Ghiasvand, R., Askari, G., Darvishi, L., Iraj, B., Mashhadi, N.S. (2013). Dietary behaviors and nutritional assessment of young male isfahani wrestlers. International Journal of Preventive Medicine. 4(1):48-52.
21. Fujii, T., Matsuo, T., Okamura, K. (2014). Effects of resistance exercise on iron absorption and balance in iron-deficient rats. Biological Trace Element Research. 161(1):101-6.
22. Qian, Z.M., Liao, Q.K., Ho, K.P. (2002). Effect of different durations of exercise on transferrin-bound iron uptake by rat erythroblast. The Journal of Nutritional Biochemistry. 13(1):47-54.
23. Neilands, J.B. (2014). Microbial Iron Metabolism: A Comprehensive Treatise. Academic Press. 28.
24. Liu, Y.Q., Chang, Y.Z., Zhao, B., Wang, H.T., Duan, X.L. (2011). Does hepatic hepcidin play an important role in exercise-associated anemia in rats? International Journal of Sport Nutrition and Exercise Metabolism. 21(1):19-26.
25. Waller, M.F., Haymes, E.M. (1996). The effects of heat and exercise on sweat iron loss. Medicine and Science in Sports and Exercise. 28(2):197-203.
26. Adams, W.D., Wild, F., Hill, R.M. (2001). Hematologic concerns in the runner. Test Book of Running Medicine. PP: 395.