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تاثیریک دوره تمرین تناوبی شدید مادری بر بیان ژن PGC1α و SIRT1 عضله اسکلتی نسل اول موش هایی صحرائی

مقالات آماده انتشار

نویسندگان

هادی حبیبی 1
علی اصغر رواسی 2
ندا خالدی 1

1 دانشگاه خوارزمی

2 دانشگاه تهران

چکیده
تاثیر فعالیت بدنی مادر بر سلامت فرزندان و تغیر فنوتیپ فرزندان در اثر ورزش یکی از موضوعات نوین فیزیولوژی ورزشی در سال های اخیر بوده که مورد توجه بسیاری قرار گرفته است. بنابراین، هدف از این پژوهش تعیین تاثیر یک دوره تمرین تناوبی شدید مادری بر بیان ژن های PGC1α و SIRT1 عضله اسکلتی نسل اول موش های صحرائی بود. 24 سر رت نژاد ویستار 8 هفته ای پس از آشنایی با محیط در سه گروه کنترل مادری، گروه مادری تمرین پیش از بارداری و گروه مادری تمرین پیش و در حین بارداری تقسیم شدند. تمرین پیش از بارداری مدت 6 هفته و تمرین در حین بارداری 3 هفته طول کشید. تمرین شامل دویدن روی تردمیل بود. تمرین شامل 5 روز و هر روز 1 دقیقه با شدت 85 الی 100 درصد vo2peak و شیب 10 درجه و دو دقیقه استراحت بین وهله ها با 65 درصد vo2 peak و شیب صفر بود و گروه کنترل در این مدت بی تحرک بودند. تعداد وهله ها از 10وهله شروع شد و بر اساس اصل اضافه بار افزایش داشت. پس از پایان دوره تمرین و به دنیا آمدن فرزندان، فرزندان نر بر اساس گروه مادران خود تقسیم بندی شدند و میزان بیان ژن های PGC1 و SIRT1 در عضله آن ها در ده هفتگی مورد ارزیابی قرار گرفت. برای تحلیل داده ها از ANOVA و آزمون تعقیبی توکی استفاده شد.

نتایج نشان داد که بعد از دوره ی 6 هفته ای تمرین HIIT، گروه تمرین پیش از بارداری و گروه تمرین پیش و حین بارداری در سرعت و مسافت در تست عملکردی با گروه کنترل اختلاف معنی دار داشتند (05/0 ˂P). همچنین بعد از تولد توله ها بین گروه ها در بیان ژن PGC1α و SIRT1 تفاوت معنی داری وجود داشت. که این تفاوت بین دو گروه تمرین پیش از بارداری و تمرین پیش از بارداری و حین بارداری با گروه کنترل معنی دار بود (05/0 ˂P). به نظر می رسد ورزش مادری در پیش از بارداری و در دوران بارداری باعث تغییر ژنوتیپ میتوکندریایی فرزندان می شود که تاثیر آن در پیش و هنگام بارداری نسبت به پیش از بارداری به تنهایی بر بیان ژن های میتوکندریایی مثل PGC1α و SIRT1 بیشتر است.

کلیدواژه‌ها

تمرین تناوبی شدید
تمرین شدید مادری
میتوکندری
اپی ژنتیک
سیرتوئین 1
PGC-1α

عنوان مقاله English

The effect of a period of high intensity interval maternal exercise training on expression of PGC1α and SIRT1 of skeletal muscle in offspring male rats

نویسندگان English

hadi habibi 1
Ali Asghar Ravasi 2
neda khaledi 1
1 kharazmi university
2 Tehran university
چکیده English

The impact of maternal physical activity on offspring health and phenotypic changes due to exercise has emerged as a significant area of interest in exercise physiology in recent years. Therefore, the aim of this study was to determine the effect of a maternal high-intensity interval training (HIIT) program on the expression of PGC1α and SIRT1 genes in the skeletal muscle of first-generation offspring in Wistar rats. Twenty-four 8-week-old female Wistar rats were acclimatized to the environment and then divided into three groups: a maternal control group, a maternal pre-pregnancy exercise group, and a maternal exercise group that trained both before and during pregnancy. The pre-pregnancy exercise regimen lasted 6 weeks, while the exercise during pregnancy lasted 3 weeks. The exercise protocol involved treadmill running, consisting of 5 days per week, with each session including 1 minute of running at 85-100% of VO2peak and a 10% incline, followed by 2 minutes of rest at 65% of VO2peak and 0% incline. The number of intervals started at 10 and increased based on the overload principle. The control group remained sedentary during this period. After the exercise period and the birth of the offspring, male offspring were categorized according to their maternal groups, and the expression levels of PGC1α and SIRT1 genes in their skeletal muscle were evaluated at 10 weeks of age. Data were analyzed using ANOVA and Tukey's post-hoc test.

The results indicated that after the 6-week HIIT program, both the pre-pregnancy exercise group and the group that exercised before and during pregnancy showed significant differences in speed and distance in the functional performance test compared to the control group (P < 0.05). Additionally, significant differences were observed in the expression of PGC1α and SIRT1 genes among the groups after the birth of the offspring. These differences were significant between the pre-pregnancy exercise group, the pre- and during-pregnancy exercise group, and the control group (P < 0.05). It appears that maternal exercise before and during pregnancy induces changes in the mitochondrial genotype of the offspring, with a more pronounced effect on the expression of mitochondrial genes such as PGC1α and SIRT1 when exercise occurs both before and during pregnancy compared to exercise before pregnancy alone.

کلیدواژه‌ها English

High-intensity interval training
maternal exercise
mitochondria
epigenetics
SIRT1
PGC-1α
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پژوهش در طب ورزشی و فناوری

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انتشار آنلاین از 01 آبان 1398