پژوهش در طب ورزشی و فناوری

تمرین ورزشی هوازی مادری بیان ژن PDK4 را در عضله اسکلتی تند و کند نسل اول فرزندان نر موش‌های صحرایی افزایش می‌دهد

نویسندگان

ستاره بیهقی 1
ندا خالدی 1
حسین عسکری 2

1 دانشگاه خوارزمی

2 دانشگاه شهید بهشتی

https://doi.org/10.29252/jsmt.12.1.13
چکیده
پژوهش‌های اخیر نشان دادند که محیط می‌تواند اصلاح کنندۀ ژن‌ها از طریق اپی‌ژنتیک باشد، بر همین اساس مداخلۀ ورزش می‌تواند منجر به تغییر بیان ژن در نسل‌های آینده گردد. از جمله عوامل مهم سوخت و ساز می‌توان به PDK4 که یک عامل اصلی چرخه پیروات دهیدروژناز است اشاره کرد. این پژوهش اثر تمرین ورزشی مادری را بر بیان ژن PDK4 در عضلات نعلی و باز کننده طویل انگشتان پا در فرزندان نسل اول مورد بررسی قرار داد. 20 سر موش صحرایی نر نژاد اسپراگ داولی دو ماهه در 3 گروه تمرینی قبل بارداری، حین بارداری و قبل و حین بارداری و یک گروه کنترل بدون تمرین ورزشی تقسیم شدند. گروه‌های تمرینی به ترتیب تحت پروتکل 4 ،3 و 7 هفته ای تمرین هوازی قرار گرفتند. بعد از گذشت 2 ماه از تولد نوزادان، به طور تصادفی ساده از هر گروه 6 سر موش صحرایی نر به منظور نمونه‌برداری انتخاب شدند. آزمودنی‌ها بوسیلۀ ترکیب کتامین (mg/kg 90) و زایلوزین (mg/kg 10) بیهوش و بلافاصله عضلۀ نعلی و عضله طویل بازکننده انگشتان (EDL) برای بررسی برداشته شد. سنجش بیان ژن‌ها با روش Real time PCR انجام شد. با آزمون آماری فیشر، اختلاف بین گروه‌های تمرینی و کنترل اندازه‌گیری شد. بیان ژن PDK4 فرزندان نسل اول تمامی گروه ها و عضلات به جز گروه تمرین قبل از بارداری و در عضله اسکلتی باز کننده طویل انگشتان پا، افزایش یافت. با توجه به نتایج به دست آمده به نظر می‌رسد احتمالا تمرین ورزشی مادری با افزایش بیان ژن‌های متابولیکی می‌تواند تنظیم متابولیکی نسل اول فرزندان را تحت تأثیر قرار دهد.

کلیدواژه‌ها

اپی‌ژنتیک
تنظیم متابولیکی
بیان ژن
چرخه پیروات دهیدروژناز

عنوان مقاله English

The aerobic maternal exercise increases PDK4 gene expression in slow and fast twitch muscles of rat male offspring.

نویسندگان English

Setare Beyhaghi 1
Neda Khaledi 1
Hossein Askari 2
1 Kharazmi University
2 Shahid Beheshti University
چکیده English

Recent studies indicate that environment can modify skeletal muscle gene expression and transcriptional factors in offspring genes. Therefore, exercising can lead to changes in the gene expression of the future generations. One of the important metabolic factors is PDK4, which is known as the main factor in Pyruvate Dehydrogenase Complex (PDC). This study examined the effects of maternal exercise on PDK4 expression in slow and fast twitch (soleus and EDL) muscles of male offspring. Twenty Sprague-Dawley rats were divided to three training groups: before pregnancy (TBP), during pregnancy (TDP) and before and during pregnancy (TBDP) and pregnancy without training (control) group. Training groups were running on a treadmill for four, three and seven weeks, respectively. Two months after the birth of the offspring, 6 male rats were chosen randomly from the offspring of each group. The samples were anesthetized using Ketamine (90 mg/kg) and Xylazine (10 mg/kg) and their soleus and EDL muscles were removed immediately for examine. Gene expression of offspring muscles were analyzed using real-time RT-PCR. Fisher test were used to analyze the difference between the training and the control group. PDK4 expression of offspring were significantly increased in all groups except for EDL muscle of the TBP group. According to the results, it seems likely maternal exercise training can affect the metabolic regulation in the offspring by increasing the expression of some metabolic genes.

کلیدواژه‌ها English

Gene expression
metabolic factors
epigenetics
Pyruvate Dehydrogenase Complex
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