مکمل گیری کوتاه مدت بتا هیدروکسی بتا متیل بوتیرات شاخص های آسیب عضلانی ایجاد شده طی مسابقات فوتبال را کاهش می دهد

نویسندگان

دانشگاه علامه طباطبائی

چکیده
هدف از پژوهش حاضر بررسی تأثیر مکمل بتا هیدروکسی بتا متیل بوتیرات در فرم اسید آزاد ((HMB-FA) بر شاخص های آسیب عضلانی پس از مسابقه فوتبال بود.24 فوتبالیست مرد (میانگین سنی 3±23 سال، شاخص توده بودنی 2±8/22 کیلوگرم بر متر مربع) داوطلبانه به عنوان نمونه آماری این پژوهش انتخاب و به صورت تصادفی به دو گروه 12 نفره مکمل و دارونما تقسیم شدند. دراین تحقیق آزمودنی ها در یک میکروسیکل هفتگی فوتبال با دو مسابقه شرکت کردند. آزمودنی های گروه مکمل روزانه سه گرم HMB-FA در طی میکروسیکل هفتگی فوتبال مصرف کردند. نمونه های خون در هفت مرحله (قبل از مصرف مکمل، قبل از مسابقه اول، بلافاصله بعد از مسابقه اول، 24 ساعت بعد از مسابقه اول، قبل از مسابقه دوم، بلافاصله بعد از مسابقه دوم و 24 ساعت بعد از مسابقه دوم ) گرفته شد. غلظت پلاسمایی کراتین کیناز(CK) و لاکتات دهیدروژناز(LDH) با روش الایزا اندازه گیری شد. آنالیز داده ها با استفاده از آزمون آماری فریدمن، ویلکاکسون، کروسکال-والیس و یو من ویتنی با سطح معنی داری 05/0 p انجام شد. پس از مصرف مکمل کاهش معنی دار سطح پلاسمایی لاکتات دهیدروژناز (درزمان های قبل و بعد از مسابقه اول) و کراتین کیناز( در زمان های بلافاصله بعد از مسابقه اول و قبل از مسابقه دوم ) مشاهده شد (برای همه، 05/0 p ) مصرف مکمل HMB-FA شاخص های آسیب عضلانی را کاهش داد که این برای ریکاوری از آسیب های ناشی از تمرین و مسابقات طی یک میکروسیکل مسابقه ضروری است. بعلاوه، مصرف مکمل HMB-FA نتایج دیگری نیز ممکن است داشته باشد که از جمله می توان به کاهش خستگی عصبی عضلانی، کاهش پاسخ التهابی و بازسازی سریع عضله اسکلتی اشاره کرد که نیازمند مطالعات بیشتر است.

کلیدواژه‌ها


عنوان مقاله English

Short Term HMB-FA Supplementation Decreases Muscle Damage Markers After Soccer Matches

نویسندگان English

Rasul Eslami
Navab Abnama
Allameh Tabataba’i University
چکیده English

The aim of the present study was to investigate The effect of HMB-FA supplementation on the muscle damage markers after soccer matches. 24 male soccer players (age: 23±3 years, BMI: 22.8±2 kg/m2) were voluntarily selected as the statistical sample of this study and were divided into two Suppplement and Placebo groups (n=12). In this study, subjects participated in a weekly soccer microcycle with two matches. Subjects in the supplement-training group consumed three grams of HMB-FA daily during a weekly soccer cycle. Blood samples were taken in seven times (before supplementation, before the first match, immediately after the first match, 24 hours after the first match, before the second match, immediately after the second match, 24 hours after the second match). Plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured by ELISA kits. Data analysis was performed using Friedman, Wilcoxon, Kruskal-Wallis and U Mann-Whitney tests with a significance level of p ≥ 0.05. After supplementation, a significant decrease in plasma levels of lactate dehydrogenase (before and after the first match) and creatine kinase (in the times immediately after the first match and before the second match) was observed (for all, p <0.05). Intaking HMB-FA supplement reduced the markers of muscle damage, which is essential for recovery from injuries caused by training and competition during a two race cycle. In addition, inaking HMB-FA supplement may have other effects, including reducing neuromuscular fatigue, reducing the inflammatory response, and rapid skeletal muscle regeneration, which requires further study.

کلیدواژه‌ها English

Muscle Damage
Creatine Kinase
Lactate Dihydrogenase
HMB-FA
Soccer
1.Andrzejewski, M., Podgórski, T., Kryściak, J., Chmura, P., Konefał, M., Chmura, J., ... & Pluta, B. (2021). Anabolic-catabolic hormonal responses in youth soccer players during a half-season. Research in Sports Medicine, 29(2), 141-154. [DOI:10.1080/15438627.2020.1734930]
1.Andrzejewski, M., Podgórski, T., Kryściak, J., Chmura, P., Konefał, M., Chmura, J., ... & Pluta, B. (2021). Anabolic-catabolic hormonal responses in youth soccer players during a half-season. Research in Sports Medicine, 29(2), 141-154. [DOI:10.1080/15438627.2020.1734930]
Clemente, F. M., González-Fernández, F. T., Ceylan, H. I., Silva, R., Younesi, S., Chen, Y. S., ... & Murawska-Ciałowicz, E. (2021). Blood Biomarkers Variations across the Pre-Season and Interactions with Training Load: A Study in Professional Soccer Players. Journal of Clinical Medicine, 10(23), 5576. [DOI:10.3390/jcm10235576]
Clemente, F. M., González-Fernández, F. T., Ceylan, H. I., Silva, R., Younesi, S., Chen, Y. S., ... & Murawska-Ciałowicz, E. (2021). Blood Biomarkers Variations across the Pre-Season and Interactions with Training Load: A Study in Professional Soccer Players. Journal of Clinical Medicine, 10(23), 5576. [DOI:10.3390/jcm10235576]
Correia, A. L. M., de Lima, F. D., Bottaro, M., Vieira, A., da Fonseca, A. C., & Lima, R. M. (2018). Pre-exercise β-hydroxy-β-methylbutyrate free-acid supplementation improves work capacity recovery: a randomized, double-blinded, placebo-controlled study. Applied Physiology, Nutrition, and Metabolism, (999), 1-6. (doi: 10.1139/apnm-2017-0867) [DOI:10.1139/apnm-2017-0867]
Correia, A. L. M., de Lima, F. D., Bottaro, M., Vieira, A., da Fonseca, A. C., & Lima, R. M. (2018). Pre-exercise β-hydroxy-β-methylbutyrate free-acid supplementation improves work capacity recovery: a randomized, double-blinded, placebo-controlled study. Applied Physiology, Nutrition, and Metabolism, (999), 1-6. (doi: 10.1139/apnm-2017-0867) [DOI:10.1139/apnm-2017-0867]
Dolci, F., Hart, N. H., Kilding, A. E., Chivers, P., Piggott, B., & Spiteri, T. (2020). Physical and energetic demand of soccer: a brief review. Strength & Conditioning Journal, 42(3), 70-77. [DOI:10.1519/SSC.0000000000000533]
Dolci, F., Hart, N. H., Kilding, A. E., Chivers, P., Piggott, B., & Spiteri, T. (2020). Physical and energetic demand of soccer: a brief review. Strength & Conditioning Journal, 42(3), 70-77. [DOI:10.1519/SSC.0000000000000533]
Fang, B., Kim, Y., & Choi, M. (2021, December). Effect of Cycle-Based High-Intensity Interval Training and Moderate to Moderate-Intensity Continuous Training in Adolescent Soccer Players. In Healthcare (Vol. 9, No. 12, p. 1628). Multidisciplinary Digital Publishing Institute. [DOI:10.3390/healthcare9121628]
Fang, B., Kim, Y., & Choi, M. (2021, December). Effect of Cycle-Based High-Intensity Interval Training and Moderate to Moderate-Intensity Continuous Training in Adolescent Soccer Players. In Healthcare (Vol. 9, No. 12, p. 1628). Multidisciplinary Digital Publishing Institute. [DOI:10.3390/healthcare9121628]
Holeček, M. (2017). Beta‐hydroxy‐beta‐methylbutyrate supplementation and skeletal muscle in healthy and muscle‐wasting conditions. Journal of cachexia, sarcopenia and muscle, 8(4), 529-541. doi: 10.1002/jcsm.12208 [DOI:10.1002/jcsm.12208]
Holeček, M. (2017). Beta‐hydroxy‐beta‐methylbutyrate supplementation and skeletal muscle in healthy and muscle‐wasting conditions. Journal of cachexia, sarcopenia and muscle, 8(4), 529-541. doi: 10.1002/jcsm.12208 [DOI:10.1002/jcsm.12208]
Kellmann, M. (2010). Preventing overtraining in athletes in high‐intensity sports and stress/recovery monitoring. Scandinavian journal of medicine & science in sports, 20, 95-102. (doi: 10.1111/j.1600-0838.2010.01192.x) [DOI:10.1111/j.1600-0838.2010.01192.x]
Kellmann, M. (2010). Preventing overtraining in athletes in high‐intensity sports and stress/recovery monitoring. Scandinavian journal of medicine & science in sports, 20, 95-102. (doi: 10.1111/j.1600-0838.2010.01192.x) [DOI:10.1111/j.1600-0838.2010.01192.x]
Khaitin, V., Bezuglov, E., Lazarev, A., Matveev, S., Ivanova, O., Maffulli, N., & Achkasov, E. (2021). Markers of muscle damage and strength performance in professional football (soccer) players during the competitive period. Annals of Translational Medicine, 9(2). [DOI:10.21037/atm-20-2923]
Khaitin, V., Bezuglov, E., Lazarev, A., Matveev, S., Ivanova, O., Maffulli, N., & Achkasov, E. (2021). Markers of muscle damage and strength performance in professional football (soccer) players during the competitive period. Annals of Translational Medicine, 9(2). [DOI:10.21037/atm-20-2923]
Lowery, R. P., Joy, J. M., Rathmacher, J. A., Baier, S. M., Fuller, J. C., Shelley, M. C., ... & Wilson, J. M. (2016). Interaction of beta-hydroxy-beta-methylbutyrate free acid and adenosine triphosphate on muscle mass, strength, and power in resistance trained individuals. Journal of strength and conditioning research, 30(7), 1843-1854. (doi: 10.1519/JSC.0000000000000482) [DOI:10.1519/JSC.0000000000000482]
Lowery, R. P., Joy, J. M., Rathmacher, J. A., Baier, S. M., Fuller, J. C., Shelley, M. C., ... & Wilson, J. M. (2016). Interaction of beta-hydroxy-beta-methylbutyrate free acid and adenosine triphosphate on muscle mass, strength, and power in resistance trained individuals. Journal of strength and conditioning research, 30(7), 1843-1854. (doi: 10.1519/JSC.0000000000000482) [DOI:10.1519/JSC.0000000000000482]
Martins, P., & Pedro, S. (2017). Motivational Regulations and Recovery in Olympic Wrestlers. International Journal of Wrestling Science, 7(1-2), 27-34. (doi: 10.1080/21615667.2017.1346345) [DOI:10.1080/21615667.2017.1346345]
Martins, P., & Pedro, S. (2017). Motivational Regulations and Recovery in Olympic Wrestlers. International Journal of Wrestling Science, 7(1-2), 27-34. (doi: 10.1080/21615667.2017.1346345) [DOI:10.1080/21615667.2017.1346345]
Mousavi, S., Nourshahi, M., Qarakhanlu, R., Hedayati, M., &, Akbarnejad, A. (2018). The Acute Effect of HMB-FA Supplement and Sport Activity on Some Factors that Influence Hypertrophy and Muscle Damage in Inactive Men. Journal of Sport Biosciences, (), -. doi: 10.22059 / jsb.2018.265762.1310. (in Persian)
Mousavi, S., Nourshahi, M., Qarakhanlu, R., Hedayati, M., &, Akbarnejad, A. (2018). The Acute Effect of HMB-FA Supplement and Sport Activity on Some Factors that Influence Hypertrophy and Muscle Damage in Inactive Men. Journal of Sport Biosciences, (), -. doi: 10.22059 / jsb.2018.265762.1310. (in Persian)
Nalbandian, M., & Takeda, M. (2016). Lactate as a signaling molecule that regulates exercise-induced adaptations. Biology, 5(4), 38. DOI: 10.3390/biology5040038. (in Persian) [DOI:10.3390/biology5040038]
Nalbandian, M., & Takeda, M. (2016). Lactate as a signaling molecule that regulates exercise-induced adaptations. Biology, 5(4), 38. DOI: 10.3390/biology5040038. (in Persian) [DOI:10.3390/biology5040038]
Narimani Pi Peshteh, Azadeh; Gorkhanloo, Reza and Aghayari, Azar (2012). The effect of short-term use of HMB supplement on plasma creatine phosphokinase levels after resistance training protocol in non-athlete women. Journal of Metabolism and Exercise, Volume 2, Number 2, pp. 163-149. (in Persian)
Narimani Pi Peshteh, Azadeh; Gorkhanloo, Reza and Aghayari, Azar (2012). The effect of short-term use of HMB supplement on plasma creatine phosphokinase levels after resistance training protocol in non-athlete women. Journal of Metabolism and Exercise, Volume 2, Number 2, pp. 163-149. (in Persian)
Nowakowska, A., Kostrzewa-Nowak, D., Buryta, R., & Nowak, R. (2019). Blood biomarkers of recovery efficiency in soccer players. International Journal of Environmental Research and Public Health, 16(18), 3279. [DOI:10.3390/ijerph16183279]
Nowakowska, A., Kostrzewa-Nowak, D., Buryta, R., & Nowak, R. (2019). Blood biomarkers of recovery efficiency in soccer players. International Journal of Environmental Research and Public Health, 16(18), 3279. [DOI:10.3390/ijerph16183279]
Redkva, P. E., Paes, M. R., Fernandez, R., & da-Silva, S. G. (2018). Correlation between match performance and field tests in professional soccer players. Journal of human kinetics, 62, 213. [DOI:10.1515/hukin-2017-0171]
Redkva, P. E., Paes, M. R., Fernandez, R., & da-Silva, S. G. (2018). Correlation between match performance and field tests in professional soccer players. Journal of human kinetics, 62, 213. [DOI:10.1515/hukin-2017-0171]
Silva, J. R., Ascensão, A., Marques, F., Seabra, A., Rebelo, A., & Magalhães, J. (2013). Neuromuscular function, hormonal and redox status and muscle damage of professional soccer players after a high-level competitive match. European journal of applied physiology, 113(9), 2193-2201. [DOI:10.1007/s00421-013-2633-8]
Silva, J. R., Ascensão, A., Marques, F., Seabra, A., Rebelo, A., & Magalhães, J. (2013). Neuromuscular function, hormonal and redox status and muscle damage of professional soccer players after a high-level competitive match. European journal of applied physiology, 113(9), 2193-2201. [DOI:10.1007/s00421-013-2633-8]
Silva, V. R., Belozo, F. L., Micheletti, T. O., Conrado, M., Stout, J. R., Pimentel, G. D., & Gonzalez, A. M. (2017). β-Hydroxy-β-methylbutyrate free acid supplementation may improve recovery and muscle adaptations after resistance training: A systematic review. Nutrition Research, 45, 1-9. (doi: 10.1016/j.nutres.2017.07.008) [DOI:10.1016/j.nutres.2017.07.008]
Silva, V. R., Belozo, F. L., Micheletti, T. O., Conrado, M., Stout, J. R., Pimentel, G. D., & Gonzalez, A. M. (2017). β-Hydroxy-β-methylbutyrate free acid supplementation may improve recovery and muscle adaptations after resistance training: A systematic review. Nutrition Research, 45, 1-9. (doi: 10.1016/j.nutres.2017.07.008) [DOI:10.1016/j.nutres.2017.07.008]
Soleymani Rasa, E., & Saidei, P. (2021). Dose-response of beta-hydroxy beta-methyl butyrate supplementation (HMB-FA) on muscle and liver damage indices induced by an eccentric resistance exercise session in non-athlete females. Journal of Applied Health Studies in Sport Physiology, 8(2), 34-43.
Soleymani Rasa, E., & Saidei, P. (2021). Dose-response of beta-hydroxy beta-methyl butyrate supplementation (HMB-FA) on muscle and liver damage indices induced by an eccentric resistance exercise session in non-athlete females. Journal of Applied Health Studies in Sport Physiology, 8(2), 34-43.
Tinsley, G. M., Givan, A. H., Graybeal, A. J., Villarreal, M. I., & Cross, A. G. (2018). β-Hydroxy β-methylbutyrate free acid alters cortisol responses, but not myofibrillar proteolysis, during a 24-h fast. British Journal of Nutrition, 119(5), 517-526. (doi: 10.1017/S0007114517003907) [DOI:10.1017/S0007114517003907]
Tinsley, G. M., Givan, A. H., Graybeal, A. J., Villarreal, M. I., & Cross, A. G. (2018). β-Hydroxy β-methylbutyrate free acid alters cortisol responses, but not myofibrillar proteolysis, during a 24-h fast. British Journal of Nutrition, 119(5), 517-526. (doi: 10.1017/S0007114517003907) [DOI:10.1017/S0007114517003907]