پژوهش در طب ورزشی و فناوری

اثر تعاملی شش هفته تمرین مقاومتی و مکمل یاری پروتئین وی بر بیان ژن PRAS40 و میزان هایپرتروفی عضله دوقلو در رت‌های نر نژاد ویستار

نویسندگان

فرشته شهیدی 1
حمیده احمدی 2
مجید کاشف 3

1 دانشیار گروه فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی،دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.

2 کارشناسی ارشد فیزیولوژی ورزش، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.

3 استاد گروه فیزیولوژی ورزش، گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران.

چکیده
PRAS40 در انواع بافت‌های بدن بیان و در مسیر رشد عضلانی وجود دارد. مطالعات نشان داده است که PRAS40در تنظیم رشد سلولی نقش دارد. هدف از این مطالعه تأثیر شش هفته تمرین مقاومتی و مکمل یاری پروتئین whey بر PRAS40 و هایپرتروفی عضله دوقلو رت‌های نر نژاد ویستار بود. این مطالعه تجربی که از لحاظ هدف توسعه‌ای بود در سال 1401 انجام شد 23 سر رت نر نژاد ویستار هشت هفته‌ای با وزن 250 گرم به طور تصادفی در چهار گروه: تمرین مقاومتی، تمرین مقاومتی+مکمل، شم و ‏کنترل قرار گرفتند. گروه تمرین مقاومتی و تمرین مقاومتی+مکمل دو هفته با تمرینات آشنا و شش هفته پنج روز در هفته به ‏تمرین مقاومتی بالا رفتن از نردبان لدر پرداختند در گروه تمرین+مکمل بعد از تمرینات مکمل ‏whey‏ به میزان g/kg05/2 به صورت گاواژ داده شد. در پایان با خارج کردن خون از قلب بافت عضله دو قلو خارج و به تانک ازت منتقل و برای بررسی بیان ژن ‏PRAS40‎‏ استفاده شد. شش هفته تمرین مقاومتی به همراه مکمل whey باعث کاهش معنی‌داری در بیان ژن PRAS40 و افزایش وزن عضله در گروه‌های تمرینی شد.

پروتکل تمرین مقاومتی همراه با مکمل whey از طریق سرکوب مسیر PRAS40 می‌تواند منجر به هایپرتروفی عضلانی شود و مسیرهای آنابولیک در عضله بیشتر فعال کند.

کلیدواژه‌ها

سیگنالینگ درون‌سلولی
تمرین قدرتی
پروتئین آب‌پنیر

عنوان مقاله English

The Interactive Effect Of Six Weeks Of Resistance Training And Whey Protein Supplementation On PRAS40 Gene Expression And Muscle Hypertrophy In Male Wistar Race Rats

نویسندگان English

fereshteh shahidi 1
hamide ahmadi 2
majid kashef 3
1 Associate Professor Department of Exercise Physiology, Department of Exercise Physiology, Faculty of Sports Sciences, Shahid Rajaei Teacher Training University, Tehran, Iran
2 Master's Degree of Exercise Physiology, Department of Exercise Physiology, Faculty of Sports Sciences, Shahid Rajaei Teacher Training University, Tehran, Iran.
3 Professor Department of Exercise Physiology, Department of Exercise Physiology, Faculty of Sports Sciences, Shahid Rajaei Teacher Training University, Tehran, Iran.
چکیده English

PRAS40 is expressed in all types of body tissues and is present in the muscle growth pathway and has multiple phosphorylation sites. Studies have shown that PRAS40 plays a role in regulating cell growth. The aim of this study was to evaluate the effect of six weeks of resistance training and whey protein supplementation on PRAS40 and biceps hypertrophy in male Wistar rats

Method:This developmental experimental study was carried out in 1402 year. 23 eight-week-old male Wistar rats (250 grams) were randomly divided into three groups: resistance training, resistance training+ supplement, and control. After two weeks of orientation, the training groups completed six weeks of resistance training, climbing the ladder five days a week. The training +supplement group received whey protein (2.05 g/kg dose) through gavage. 48 hours after the last training, a blood sample was taken and the gastrocnemius muscle was removed, frizzed, and saved to evaluate the expression of the PRAS40 gene.

Findings: Six weeks of resistance training with whey supplement caused a significant decrease in PRAS40 gene expression and an increase in body and muscle weight in the exercise and supplement + exercise groups.

Conclusion: The resistance training protocol along with her supplement can lead to muscle hypertrophy and activate the anabolic pathways in the muscle through the suppression of the PRAS40 pathway.

کلیدواژه‌ها English

 Intracellular Signaling
strength training
PRAS40
Whey Protein
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