Research in Sports Medicine and Technology

The effect of aerobic training along with rice bran consumption on the serum levels of some inflammatory markers of overweight men

Articles in Press

Authors

Abbas Laal Sazegar
Shahram Gholamrezaei Darsara
Mohammadreza Fadai Chafi

Department of Physical Education and Sport Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.

Abstract
Exercise training and rice bran have anti-inflammatory effects. However, the effect of combining the two interventions on inflammatory factors is unclear. Therefore, the present study aimed to investigate the effect of aerobic training and rice bran consumption on the levels of selected inflammatory factors in overweight men. Sixty overweight men were randomly divided into four groups (n=15): control, rice bran, progressive aerobic training, and training + rice bran. Progressive aerobic training was performed at an intensity of 60-75% of maximum heart rate for, 20-35 min, 8 weeks, with 3 sessions per week. Rice bran was consumed in an amount of 10 grams twice a day (before breakfast and before sleeping). Before and after the completion of the interventions, blood samples were taken and serum levels of CRP, IL-6, and TNF-α were measured by ELISA. Analysis of covariance with Bonferroni post hoc test was used to interpret the data. There were significant decreases in the levels of CRP, IL-6, and TNF-α with training and training + rice bran compared to the control and rice bran groups (p<0.05). The CRP and TNF-α reduction was also significant after rice bran consumption compared to the control group (p<0.001). Moreover, decrease in the levels of IL-6 (p=0.002) and TNF-α (p<0.001) in the training +rice bran group compared to the training group was also significant. The findings confirmed that aerobic training and rice bran consumption are effective in reducing inflammation, and there was a synergistic effect of rice bran supplementation and aerobic training.

Keywords

endurance training
IL-6
TNF-a
CRP
inflammation
References

1. Engin A. The definition and prevalence of obesity and metabolic syndrome. Obesity and lipotoxicity. 2017:1-17.
2. Rohm TV, Meier DT, Olefsky JM, Donath MY. Inflammation in obesity, diabetes, and related disorders. Immunity. 2022;55(1):31-55.
3. Wu H, Ballantyne CM. Metabolic inflammation and insulin resistance in obesity. Circulation research. 2020;126(11):1549-64.
4. Castro A, Macedo-De la Concha L, Pantoja-Meléndez C. Low-grade inflammation and its relation to obesity and chronic degenerative diseases. Revista Médica del Hospital General de México. 2017;80(2):101-5.
5. Lee H, Lee IS, Choue R. Obesity, inflammation and diet. Pediatric gastroenterology, hepatology & nutrition. 2013;16(3):143-52.
6. Cancello R, Clement K. Is obesity an inflammatory illness? Role of low‐grade inflammation and macrophage infiltration in human white adipose tissue. BJOG: An International Journal of Obstetrics & Gynaecology. 2006;113(10):1141-7.
7. Hirai S, Takahashi N, Goto T, Lin S, Uemura T, Yu R, et al. Functional food targeting the regulation of obesity‐induced inflammatory responses and pathologies. Mediators of inflammation. 2010;2010(1):367838.
8. Aggarwal BB. Targeting inflammation-induced obesity and metabolic diseases by curcumin and other nutraceuticals. Annual review of nutrition. 2010;30(1):173-99.
9. Beavers KM, Brinkley TE, Nicklas BJ. Effect of exercise training on chronic inflammation. Clinica chimica acta. 2010;411(11-12):785-93.
10. Gonzalo-Encabo P, Maldonado G, Valadés D, Ferragut C, Pérez-López A. The role of exercise training on low-grade systemic inflammation in adults with overweight and obesity: A systematic review. International journal of environmental research and public health. 2021;18(24):13258.
11. Sakurai T, Ogasawara J, Kizaki T, Sato S, Ishibashi Y, Takahashi M, et al. The effects of exercise training on obesity‐induced dysregulated expression of adipokines in white adipose tissue. International journal of endocrinology. 2013;2013(1):801743.
12. Dasgupta A. Antiinflammatory herbal supplements. Translational inflammation: Elsevier; 2019. p. 69-91.
13. Park H-Y, Lee K-W, Choi H-D. Rice bran constituents: Immunomodulatory and therapeutic activities. Food & function. 2017;8(3):935-43.
14. Kurtys E, Eisel UL, Hageman RJ, Verkuyl JM, Broersen LM, Dierckx RA, et al. Anti-inflammatory effects of rice bran components. Nutrition reviews. 2018;76(5):372-9.
15. Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P. Rice bran protein hydrolysates improve insulin resistance and decrease pro-inflammatory cytokine gene expression in rats fed a high carbohydrate-high fat diet. Nutrients. 2015;7(8):6313-29.
16. Cannon J, Micalos P, Pak S. A study protocol to evaluate a fermented rice bran supplement and resistance training on immune function and muscle performance in healthy older people. Journal of Science and Medicine in Sport. 2019;22:S79.
17. Tazakori Z, Dehghan M, Iranparvar M, Zare M, Foladi N, Mohmmadi R. Effect of rice bran powder on blood glucose levels and serum lipid parameters in diabetes patient II. 2007.
18. Wilund KR. Is the anti-inflammatory effect of regular exercise responsible for reduced cardiovascular disease? Clinical science. 2007;112(11):543-55.
19. Bruunsgaard H. Physical activity and modulation of systemic low-level inflammation. Journal of leukocyte biology. 2005;78(4):819-35.
20. Hotamisligil GS. Inflammation, metaflammation and immunometabolic disorders. Nature. 2017;542(7640):177-85.
21. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. The Journal of clinical investigation. 2003;111(12):1805-12.
22. Chandrashekara S. C-reactive protein: An inflammatory marker with specific role in physiology, pathology, and diagnosis. Internet Journal of Rheumatology and Clinical Immunology. 2014;2(S1):01-23.
23. Lehrskov LL, Christensen RH, editors. The role of interleukin-6 in glucose homeostasis and lipid metabolism. Seminars in immunopathology; 2019: Springer.
24. Horiuchi T, Mitoma H, Harashima S-i, Tsukamoto H, Shimoda T. Transmembrane TNF-α: structure, function and interaction with anti-TNF agents. Rheumatology. 2010;49(7):1215-28.
25. Park HS, Park JY, Yu R. Relationship of obesity and visceral adiposity with serum concentrations of CRP, TNF-α and IL-6. Diabetes research and clinical practice. 2005;69(1):29-35.
26. Mohamed-Ali V, Goodrick S, Rawesh A, Katz D, Miles J, Yudkin J, et al. Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor-α, in vivo. The Journal of Clinical Endocrinology & Metabolism. 1997;82(12):4196-200.
27. Hotamisligil GS, Erbay E. Nutrient sensing and inflammation in metabolic diseases. Nature Reviews Immunology. 2008;8(12):923-34.
28. Sun K, Kusminski CM, Scherer PE. Adipose tissue remodeling and obesity. The Journal of clinical investigation. 2011;121(6):2094-101.
29. Sakurai T, Ogasawara J, Shirato K, Izawa T, Oh-Ishi S, Ishibashi Y, et al. Exercise training attenuates the dysregulated expression of adipokines and oxidative stress in white adipose tissue. Oxidative medicine and cellular longevity. 2017;2017(1):9410954.
30. Vijayaraghava A, Doreswamy V. Exercise and the cytokines-interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha): A review: Exercise and the cytokines, IL-6, TNF-alpha. Annals of Medical Physiology. 2017;1(1):3-8.
31. Abd El-Kader SM. Aerobic versus resistance exercise training in modulation of insulin resistance, adipocytokines and inflammatory cytokine levels in obese type 2 diabetic patients. Journal of Advanced research. 2011;2(2):179-83.
32. Pranoto A, Cahyono MBA, Yakobus R, Izzatunnisa N, Ramadhan RN, Rejeki PS, et al. Long-term resistance–endurance combined training reduces pro-inflammatory cytokines in young adult females with obesity. Sports. 2023;11(3):54.
33. Fisher G, Hyatt TC, Hunter GR, Oster RA, Desmond RA, Gower BA. Effect of diet with and without exercise training on markers of inflammation and fat distribution in overweight women. Obesity. 2011;19(6):1131-6.
34. Balducci S, Zanuso S, Nicolucci A, Fernando F, Cavallo S, Cardelli P, et al. Anti-inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nutrition, Metabolism and Cardiovascular Diseases. 2010;20(8):608-17.
35. Woods JA, Vieira VJ, Keylock KT. Exercise, inflammation, and innate immunity. Immunology and allergy clinics of North America. 2009;29(2):381-93.
36. You T, Arsenis NC, Disanzo BL, LaMonte MJ. Effects of exercise training on chronic inflammation in obesity: current evidence and potential mechanisms. Sports Medicine. 2013;43:243-56.
37. Sivamaruthi BS, Alagarsamy K, Thangaleela S, Bharathi M, Kesika P, Chaiyasut C. Composition, microbiota, mechanisms, and anti-obesity properties of Rice bran. Foods. 2023;12(6):1300.
38. Edrisi F, Salehi M, Ahmadi A, Fararoei M, Rusta F, Mahmoodianfard S. Effects of supplementation with rice husk powder and rice bran on inflammatory factors in overweight and obese adults following an energy-restricted diet: a randomized controlled trial. European journal of nutrition. 2018;57:833-43.
39. Papathanasopoulos A, Camilleri M. Dietary fiber supplements: effects in obesity and metabolic syndrome and relationship to gastrointestinal functions. Gastroenterology. 2010;138(1):65-72. e2.
40. Candiracci M, Justo ML, Castano A, Rodriguez-Rodriguez R, Herrera MD. Rice bran enzymatic extract–supplemented diets modulate adipose tissue inflammation markers in Zucker rats. Nutrition. 2014;30(4):466-72.
41. King DE. Dietary fiber, inflammation, and cardiovascular disease. Molecular nutrition & food research. 2005;49(6):594-600.
42. Galisteo M, Duarte J, Zarzuelo A. Effects of dietary fibers on disturbances clustered in the metabolic syndrome. The Journal of nutritional biochemistry. 2008;19(2):71-84.
43. Lee S, Yu S, Park HJ, Jung J, Go G-w, Kim W. Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages. PloS one. 2019;14(10):e0222857.
44. Shen J, Yang T, Xu Y, Luo Y, Zhong X, Shi L, et al. δ-Tocotrienol, isolated from rice bran, exerts an anti-inflammatory effect via MAPKs and PPARs signaling pathways in lipopolysaccharide-stimulated macrophages. International journal of molecular sciences. 2018;19(10):3022.
45. Moazamigoodarzi M, Gholamrezaei Darsara S, ََAzarbayjani MA, Elmiye A. The Simultaneous Effect of Aerobic Exercise and Rice Bran Extract Consumption on Inflammatory Genes Expression in White Adipose Tissue of Obese Female Rats. Journal of Sport Biosciences. 2023;15(4):5-20.
46. Moazami Goodarzi M, Gholamrezaei S, Azarbayjani MA, Elmieh A. The effect of rice bran extract and aerobic exercise on oxidative stress indices of white adipose tissue in obese rats. Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch. 2023;33(4):347-54.
Research in Sports Medicine and Technology

Articles in Press, Accepted Manuscript
Available Online from 23 October 2019