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تاثیر 8 هفته فعالیت ورزشی تدامی با شدت متوسط بر بیان پروتئین های IL-10 و HMGB1 و فعالیت آنزیم های SOD و GPx در هیپوکمپ رت های مبتلا به صرع

مقالات آماده انتشار

نویسندگان

یونس سرکبود
محمدرضا کردی
سیروس چوبینه
Fatemeh Nasery

دانشگاه تهران

چکیده
مقدمه و هدف: استرس اکسیداتیو و التهاب عصبی از مکانیسم‌های کلیدی در پاتوفیزیولوژی بیماری صرع هستند. فعالیت ورزشی در کنترل و بهبود علائم صرع نقش دارد. این مطالعه با هدف بررسی اثر تمرینات تداومی با شدت متوسط (MICT) بر فعالیت آنزیم­های آنتی­اکسیدانی سوپراکسیددیسموتاز (SOD) وگلوتاتیون پراکسیداز (GPx)، سطوح پروتئین­های HMGB1 و IL-10 و شدت تشنج در هیپوکمپ رت­های مبتلا به صرع انجام شد.

روش: 32 سر رت نر ویستار (سن: 6 تا 8 هفته و میانگین وزن: ۲۲۶٫۶۲۵±۱۴٫۹۶۶) به گروه‌های صرع، کنترل، شم و صرع + MICT تقسیم شدند. پس از اتمام پروتکل هشت هفته­ای تمرین، فعالیت SOD و GPx با الایزا، سطوح HMGB1 و IL-10 در بافت هیپوکمپ با وسترن­بلات و شدت تشنج بر اساس مقیاس راسین اندازه گیری شد. داده­ها از طریق آزمون تحلیل واریانس یک­راهه و آزمون یومن­ویتنی در نرم­افزار SPSS تجزیه و تحلیل شدند.

یافته‌­ها: القای صرع منجر به کاهش فعالیت آنزیم‌های SOD (0.02p < ) و GPx (0.001p < ) ، افزایش پروتئینHMGB1 (0.001p < ) ، کاهش پروتئین IL-10(0.001p < ) و افزایش شدت تشنج شد. تمرین MICT موجب افزایش فعالیت آنزیم GPx (0.048p < ) و کاهش سطح HMGB1 (0.001p < ) در گروه تمرینی نسبت به گروه صرع شد. اگرچه فعالیت آنزیم SOD (0.953p < ) و سطح IL-10 (0.198p < ) تغییر معنا‌داری نیافت، اما شدت تشنج در گروه تحت تمرین به طور قابل توجهی کاهش یافت.

نتیجه‌گیری: به نظر می‌رسد تمرینات تداومی با شدت متوسط با تقویت سیستم آنتی‌اکسیدانی و کاهش التهاب عصبی، اثرات محافظتی خود را اعمال کرده و باعث کاهش شدت تشنج در رت­های مبتلا به صرع می­شود.

کلیدواژه‌ها

صرع
فعالیت ورزشی تداومی با شدت متوسط
التهاب
آنتی­ اکسیدان
تشنج

عنوان مقاله English

The Effect of Eight Weeks of Moderate-Intensity Continuous Exercise on the Expression of IL-10 and HMGB1 Proteins and the Activity of SOD and GPx Enzymes in the Hippocampus of Epileptic Rat

نویسندگان English

Younes Sarkabood
Mohamadreza Kordi
Siroos Choobineh
Fatemeh Nasery
University of Tehran
چکیده English

Abstract

Introduction: Oxidative stress and neuroinflammation are key mechanisms in the pathophysiology of epilepsy. Physical activity plays a role in controlling and improving epileptic symptoms. This study aimed to investigate the effects of moderate-intensity continuous training (MICT) on the activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), the levels of HMGB1 and IL-10 proteins, and seizure severity in the hippocampus of epileptic rats.

Methods: Thirty-two male Wistar rats (6–8 weeks old; mean body weight: 226.625 ± 14.966 g) were randomly assigned to four groups: epilepsy, control, sham, and epilepsy + MICT. After completion of the 8-week training protocol, SOD and GPx activities were measured by ELISA, hippocampal HMGB1 and IL-10 levels were assessed by Western blotting, and seizure severity was evaluated using the Racine scale. Data were analyzed using one-way analysis of variance (ANOVA) and the Mann–Whitney U test in SPSS.

Results: Epilepsy induction led to decreased SOD (p < 0.02) and GPx (p < 0.001) activities, increased HMGB1 levels (p < 0.001), decreased IL-10 levels (p < 0.001), and increased seizure severity. MICT increased GPx activity (p < 0.048) and reduced HMGB1 levels (p < 0.001) in the training group compared with the epilepsy group. Although SOD activity (p = 0.953) and IL-10 levels (p = 0.198) did not change significantly, seizure severity was significantly reduced in the trained group.

Conclusion: Moderate-intensity continuous training appears to exert protective effects and reduce seizure severity in epileptic rats by strengthening the antioxidant defense system and attenuating neuroinflammation.

کلیدواژه‌ها English

Epilepsy
Moderate-Intensity Continuous Training
inflammation
antioxidant
Seizure
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پژوهش در طب ورزشی و فناوری

مقالات آماده انتشار، پذیرفته شده
انتشار آنلاین از 01 آبان 1398