Research in Sports Medicine and Technology

Investigating the relationship between ACTN3 rs1815739 and COL5A1 rs12722 polymorphisms with power performance following plyometric exercises

Articles in Press

Authors

mohsen Alinaghizade 1
Reza Gharakhanlou 1
Mahdieh Mollanouri Shamsi 2

1 Department of Exercise Physiology, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran

2 Faculty of Psychology and Educational Sciences, Department of Sports Sciences, Yazd University, Yazd, Iran.

Abstract
Introduction

previous research has shown that ACTN3 rs1815739 and COL5A1 rs12722 polymorphisms may affect power performance. This research aimed to investigate the relationship between ACTN3 and COL5A1 polymorphisms with the baseline level of power performance and its effect on adaptation to plyometric training.

Methods

The research subjects were 38 boys with an average age of 10.3 ± 0.53. Vertical jump (CMJ) and horizontal jump (SBJ) tests were used to evaluate lower body muscle power. The plyometric training protocol was for 6 weeks. DNA was extracted from saliva and genotype was determined by PCR-RFLP method. Based on genetic analysis, people were divided into the following groups in the ACTN3 polymorphism: RX (n=20), RR (n=13), and XX (n=5), and in the COL5A1 polymorphism: CT (n=19), CC (n =8) and TT (n=11). ANCOVA test was used for statistical analysis.

Results

The results showed that plyometric training caused a significant increase in CMJ and SBJ tests (p<0.05). However, no significant difference was observed when the average basic performance and adaptation to plyometric training were compared according to ACTN3 and COL5A1 genotypes (p>0.05).

Conclusion

plyometric training effectively increases power in children, but these differences are probably not affected by ACTN3 and COL5A1 genotypes.

Keywords

Power
plyometrics
genetics
ACTN3
COL5A1
[1] T. J. Suchomel, S. Nimphius, and M. H. Stone, “The Importance of Muscular Strength in Athletic Performance,” Sport. Med. 2016 4610, vol. 46, no. 10, pp. 1419–1449, Feb. 2016, doi: 10.1007/S40279-016-0486-0.
[2] G. Davies, B. L. Riemann, and R. Manske, “CURRENT CONCEPTS OF PLYOMETRIC EXERCISE.,” Int. J. Sports Phys. Ther., vol. 10, no. 6, pp. 760–86, Nov. 2015.
[3] C. Bouchard and E. P. Hoffman, Genetic and Molecular Aspects of Sport Performance. Wiley-Blackwell, 2010. doi: 10.1002/9781444327335.
[4] C. Bouchard et al., “Familial aggregation of V̇O(2max) response to exercise training: Results from the HERITAGE family study,” J. Appl. Physiol., vol. 87, no. 3, pp. 1003–1008, Sep. 1999, doi: 10.1152/jappl.1999.87.3.1003.
[5] M. A. Sarzynski, T. Rankinen, and C. Bouchard, “Twin and Family Studies of Training Responses,” in Genetic and Molecular Aspects of Sport Performance, 2010, pp. 110–120. doi: 10.1002/9781444327335.ch10.
[6] M. J. Hubal et al., “Variability in muscle size and strength gain after unilateral resistance training,” Med. Sci. Sport. Exerc., vol. 37, no. 6, pp. 964–972, 2005.
[7] R. S. Lloyd, J. M. Radnor, M. B. A. De Ste Croix, J. B. Cronin, and J. L. Oliver, “Changes in sprint and jump performances after traditional, plyometric, and combined resistance training in male youth pre- and post-peak height velocity,” J. Strength Cond. Res., vol. 30, no. 5, pp. 1239–1247, May 2016, doi: 10.1519/JSC.0000000000001216.
[8] E. A. Semenova, E. C. R. Hall, and I. I. Ahmetov, “Genes and Athletic Performance: The 2023 Update,” Genes (Basel)., vol. 14, no. 6, Jun. 2023, doi: 10.3390/GENES14061235.
[9] C. McGlory and S. M. Phillips, “Exercise and the Regulation of Skeletal Muscle Hypertrophy,” in Progress in Molecular Biology and Translational Science, Elsevier B.V., 2015, pp. 153–173. doi: 10.1016/bs.pmbts.2015.06.018.
[10] J. Del Coso, D. Hiam, P. Houweling, L. M. Pérez, N. Eynon, and A. Lucía, “More than a ‘speed gene’: ACTN3 R577X genotype, trainability, muscle damage, and the risk for injuries,” European Journal of Applied Physiology, vol. 119, no. 1. Springer Verlag, pp. 49–60, Jan. 30, 2019. doi: 10.1007/s00421-018-4010-0.
[11] I. I. Ahmetov and O. N. Fedotovskaya, Current Progress in Sports Genomics, 1st ed., vol. 70. Elsevier Inc., 2015. doi: 10.1016/bs.acc.2015.03.003.
[12] C. Pickering and J. Kiely, “ACTN3: More than just a gene for speed,” Frontiers in Physiology, vol. 8, no. DEC. Frontiers Media S.A., Dec. 18, 2017. doi: 10.3389/fphys.2017.01080.
[13] N. Eynon, J. R. Ruiz, J. Oliveira, J. A. Duarte, R. Birk, and A. Lucia, “Genes and elite athletes: a roadmap for future research,” J. Physiol., vol. 589, no. 13, pp. 3063–3070, Jul. 2011, doi: 10.1113/jphysiol.2011.207035.
[14] S. Broos, M. Van Leemputte, L. Deldicque, and M. A. Thomis, “History-dependent force, angular velocity and muscular endurance in ACTN3 genotypes,” Eur. J. Appl. Physiol., vol. 115, no. 8, pp. 1637–1643, Aug. 2015, doi: 10.1007/s00421-015-3144-6.
[15] C. Pickering and J. Kiely, “ACTN3, morbidity, and healthy aging,” Frontiers in Genetics, vol. 9, no. JAN. Frontiers Media S.A., Jan. 24, 2018. doi: 10.3389/fgene.2018.00015.
[16] J. C. Brown, C. J. Miller, M. P. Schwellnus, and M. Collins, “Range of motion measurements diverge with increasing age for COL5A1 genotypes,” Scand. J. Med. Sci. Sport., vol. 21, no. 6, Dec. 2011, doi: 10.1111/j.1600-0838.2010.01271.x.
[17] A. D. Faigenbaum et al., “Youth resistance training: updated position statement paper from the national strength and conditioning association.,” Journal of strength and conditioning research / National Strength & Conditioning Association, vol. 23, no. 5 Suppl. 2009. doi: 10.1519/jsc.0b013e31819df407.
[18] K. Silventoinen, P. K. E. Magnusson, P. Tynelius, J. Kaprio, and F. Rasmussen, “Heritability of body size and muscle strength in young adulthood: A study of one million Swedish men,” Genet. Epidemiol., vol. 32, no. 4, pp. 341–349, May 2008, doi: 10.1002/gepi.20308.
[19] T. J. Lohman, A. F. Roache, and R. Martorell, “Anthropometric Standardization Reference Manual,” Med. Sci. Sport. Exerc., vol. 24, no. 8, p. 952, 1992, doi: 10.1249/00005768-199208000-00020.
[20] J. F. Glatthorn, S. Gouge, S. Nussbaumer, S. Stauffacher, F. M. Impellizzeri, and N. A. Maffiuletti, “Validity and reliability of optojump photoelectric cells for estimating vertical jump height,” J. Strength Cond. Res., vol. 25, no. 2, pp. 556–560, 2011, doi: 10.1519/JSC.0b013e3181ccb18d.
[21] C. Balsalobre-Fernández, C. M. Tejero-González, J. Del Campo-Vecino, and N. Bavaresco, “The concurrent validity and reliability of a low-cost, high-speed camera-based method for measuring the flight time of vertical jumps,” J. Strength Cond. Res., vol. 28, no. 2, pp. 528–533, 2014, doi: 10.1519/JSC.0b013e318299a52e.
[22] J. M. González-Ravé, L. Machado, F. Navarro-Valdivielso, and J. P. Vilas-Boas, “Acute effects of heavy-load exercises, stretching exercises, and heavy-load plus stretching exercises on squat jump and countermovement jump performance,” J. Strength Cond. Res., vol. 23, no. 2, pp. 472–479, 2009, doi: 10.1519/JSC.0b013e318198f912.
[23] C. Adam, V. Klissouras, M. Ravazzolo, R. Renson, and W. Tuxworth, “EUROFIT: European test of physical fitness,” Rome Counc. Eur. Comm. Dev. Sport, pp. 10–70, 1988, doi: 10.1109/ICRA.2015.7139455.
[24] M. R. Goode, S. Y. Cheong, N. Li, W. C. Ray, and C. W. Bartlett, “Collection and extraction of saliva DNA for next generation sequencing,” J. Vis. Exp., no. 90, Aug. 2014, doi: 10.3791/51697.
[25] J. M. Radnor, R. S. Lloyd, and J. L. Oliver, “Individual Response to Different Forms of Resistance Training in School-Aged Boys,” J. Strength Cond. Res., vol. 31, no. 3, pp. 787–797, Mar. 2017, doi: 10.1519/JSC.0000000000001527.
[26] R. S. Lloyd et al., “Position statement on youth resistance training: The 2014 International Consensus,” Br. J. Sports Med., vol. 48, no. 7, pp. 498–505, 2014, doi: 10.1136/bjsports-2013-092952.
[27] E. S. S. de Villarreal, B. Requena, and R. U. Newton, “Does plyometric training improve strength performance? A meta-analysis,” Journal of Science and Medicine in Sport, vol. 13, no. 5. pp. 513–522, 2010. doi: 10.1016/j.jsams.2009.08.005.
[28] M. C. Rumpf, J. B. Cronin, S. D. Pinder, J. Oliver, and M. Hughes, “Effect of different training methods on running sprint times in male youth,” Pediatr. Exerc. Sci., vol. 24, no. 2, pp. 170–186, 2012, doi: 10.1123/pes.24.2.170.
[29] E. S. De Villarreal, B. Requena, and J. B. Cronin, “The effects of plyometric training on sprint performance: A meta-analysis,” J. Strength Cond. Res., vol. 26, no. 2, pp. 575–584, Feb. 2012, doi: 10.1519/JSC.0b013e318220fd03.
[30] L. L. Chiu, Y. F. Wu, M. T. Tang, H. C. Yu, L. L. Hsieh, and S. S. Y. Hsieh, “ACTN3 genotype and swimming performance in Taiwan,” Int. J. Sports Med., vol. 32, no. 6, pp. 476–480, 2011, doi: 10.1055/s-0030-1263115.
[31] C. N. Moran et al., “Association analysis of the ACTN3 R577X polymorphism and complex quantitative body composition and performance phenotypes in adolescent Greeks,” Eur. J. Hum. Genet., vol. 15, no. 1, pp. 88–93, 2007, doi: 10.1038/sj.ejhg.5201724.
[32] L. L. Chiu, T. W. Chen, S. S. Hsieh, and L. L. Hsieh, “ACE I/D, ACTN3 R577X, PPARD T294C and PPARGC1A Gly482Ser polymorphisms and physical fitness in Taiwanese late adolescent girls,” J. Physiol. Sci., vol. 62, no. 2, pp. 115–121, Mar. 2012, doi: 10.1007/s12576-011-0189-0.
[33] P. Gentil, R. W. Pereira, T. K. M. Leite, and M. Bottaro, “ACTN3 R577X polymorphism and neuromuscular response to resistance training,” J. Sport. Sci. Med., vol. 10, no. 2, pp. 393–399, 2011.
[34] J. Orysiak et al., “Relationship between ACTN3 R577X polymorphism and maximal power output in elite Polish athletes,” Med. 2014, Vol. 50, Pages 303-308, vol. 50, no. 5, pp. 303–308, Oct. 2014, doi: 10.1016/J.MEDICI.2014.10.002.
[35] C. N. Moran et al., “Association analysis of the ACTN3 R577X polymorphism and complex quantitative body composition and performance phenotypes in adolescent Greeks,” Eur. J. Hum. Genet., vol. 15, no. 1, pp. 88–93, 2007.
[36] P. Stastny et al., “Effect of COL5A1, GDF5, and PPARA Genes on a Movement Screen and Neuromuscular Performance in Adolescent Team Sport Athletes,” J. Strength Cond. Res., vol. 33, no. 8, pp. 2057–2065, Aug. 2019, doi: 10.1519/JSC.0000000000003142.
[37] C. F. Murtagh et al., “The genetic profile of elite youth soccer players and its association with power and speed depends on maturity status,” PLoS One, vol. 15, no. 6, p. e0234458, Jun. 2020, doi: 10.1371/JOURNAL.PONE.0234458.
[38] I. I. Ahmetov, E. S. Egorova, L. J. Gabdrakhmanova, and O. N. Fedotovskaya, “Genes and athletic performance: an update,” in Genetics and Sports, vol. 61, Karger Publishers, 2016, pp. 41–54.
Research in Sports Medicine and Technology

Articles in Press, Accepted Manuscript
Available Online from 23 October 2019